Vitamins & Minerals

Vitamin D (25-OH)

Vitamin D (25-hydroxyvitamin D) is a fat-soluble hormone precursor synthesised in the skin upon UVB exposure and obtained through diet. It plays a critical role in calcium absorption, immune regulation, and gene expression across hundreds of pathways. Blood levels of 25-OH-D are the gold standard for assessing vitamin D status.

Optimal Ranges

Clinical (NHS) Range

30-100 nmol/L

nmol/L

Performance-Optimised Range

100-150 nmol/L

nmol/L

The clinical range defines what is considered medically “normal” — broad enough to cover 95% of the population. The performance range reflects where research and clinical experience suggest most people feel and function at their best.

Why It Matters

Why Vitamin D matters for performance

Vitamin D is one of the most common deficiencies in the UK, particularly during autumn and winter when UVB exposure drops to near zero. For men focused on performance, suboptimal vitamin D is linked to reduced testosterone production, impaired muscle protein synthesis, and slower recovery from training. Studies consistently show that men with levels above 40 ng/mL have higher free testosterone and better muscular function than those in the low-normal range.

Symptoms

Signs your levels may be off

Low / Deficiency

  • Fatigue and low energy
  • Muscle weakness and aches
  • Low mood and seasonal depression
  • Frequent illness and slow recovery
  • Bone pain and stress fractures

High / Excess

  • Nausea and vomiting
  • Kidney stones
  • Hypercalcaemia (elevated calcium)
  • Confusion and disorientation

Dietary Sources

Foods that support Vitamin D levels

Oily fish (salmon, mackerel, sardines)Egg yolksFortified dairy and plant milksLiverMushrooms exposed to UV light

Supplementation

Evidence-based supplementation approach

Vitamin D3 (cholecalciferol) is the preferred form, dosed at 1,000-4,000 IU daily depending on baseline levels. Co-supplementation with vitamin K2 (MK-7) is recommended to direct calcium into bones rather than soft tissue. Fat-soluble — take with a meal containing dietary fat for optimal absorption. Retest at 90 days to confirm response.

Research

Key study

Effect of vitamin D supplementation on testosterone levels in men

Pilz S, Frisch S, Koertke H, et al.

Hormone and Metabolic Research (2011)

DOI: 10.1055/s-0030-1269854

Related Biomarkers

Related Guides

Test your Vitamin D levels

Vitamin D is included in the Helvy 50+ biomarker panel. Get your results in 5 days with a personalised protocol.

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This content is for educational purposes only and does not constitute medical advice. Your data suggests areas for optimisation, but any concerns should be discussed with a qualified healthcare professional. If your results flag values outside safe ranges, we recommend consulting your GP.